Article Dans Une Revue Pharmaceuticals Année : 2025

Enhancement of In Vivo Bone Regeneration by the Carbohydrate Derivative DP2

Résumé

Background/Objectives: Delays in bone healing and complications of remodeling constitute a major medical problem—particularly in older adults and patients with comorbidities. Current therapeutic approaches are based on strategies that promote bone regeneration. We recently identified a disaccharide compound (DP2) that enhances in vitro mineralization in human osteoblast cells via the early activation of Runx2 and the induction of osteoblast differentiation.
Methods: First, a calcium quantification assay was performed to assess mineralization in MC3T3-E1 cells. Next, microcomputed tomography and histological analyses were used to examine in vivo bone repair in a rat 5 mm cranial defect model following the implantation of DP2 coupled to a micro/macroporous biphasic CaP ceramic (MBCP+) or collagen scaffold.
Results: Here, we demonstrated that DP2 induced osteogenic differentiation and significantly elevated calcium matrix deposition in the murine preosteoblast cell line MC3T3-E1. We found that treatment with DP2 coupled to MBCP+ repaired the calvarial defect on post-implantation day 91. It significantly increased bone mineral density starting on day 29 post-treatment. In addition, DP2 did not induce ectopic bone formation.
Conclusion: Taken as a whole, these results show that DP2 is a promising candidate treatment for delayed bone healing.
Fichier principal
Vignette du fichier
pharmaceuticals-18-00215-v2.pdf (3) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
licence

Dates et versions

hal-04939770 , version 1 (12-02-2025)

Licence

Identifiants

Citer

Nissrine Ballout, Sylvestre Toumieux, Walaa Darwiche, Cathy Gomila, Eric Trécherel, et al.. Enhancement of In Vivo Bone Regeneration by the Carbohydrate Derivative DP2. Pharmaceuticals, 2025, 18 (2), pp.215. ⟨10.3390/ph18020215⟩. ⟨hal-04939770⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More