%0 Journal Article %T Analysis of Mesencephalic Astrocyte-derived Neurotrophic Factor in Multiple Myeloma %+ CHU Amiens-Picardie %+ Centre Eugène Marquis (CRLCC) %+ Chemistry, Oncogenesis, Stress and Signaling (COSS) %+ Service de Médecine Interne [CHU Amiens-Picardie] %+ Réseau d'Epidémiologie Clinique International Francophone [Amiens] (RECIF Amiens) %+ CHirurgie, IMagerie et REgénération tissulaire de l’extrémité céphalique - Caractérisation morphologique et fonctionnelle - UR UPJV 7516 (CHIMERE) %+ HEMATIM - Hématopoïèse et immunologie - UR UPJV 4666 (HEMATIM) %+ Service de dermatologie [CHU d'Amiens-Picardie] %+ Mécanismes physiopathologiques et conséquences des calcifications vasculaires - UR UPJV 7517 (MP3CV) %+ Biobanque de Picardie [CHU Amiens - Hôpital Sud] %A Dernoncourt, Amandine %A Sauzay, Chloé %A Schmidt, Jean %A Louandre, Christophe %A Gomila, Cathy %A Duhaut, Pierre %A Herpe, Yves-Edouard %A Saidak, Zuzana %A Galmiche, Antoine %A Salle, Valery %< avec comité de lecture %@ 0250-7005 %J Anticancer Research %I International Institute of Anticancer Research %V 41 %N 9 %P 4305-4312 %8 2021-09 %D 2021 %R 10.21873/anticanres.15235 %M 34475050 %K Multiple myeloma %K biomarker %K cancer. %K mesencephalic astrocyte-derived neurotrophic factor %K unfolded protein response %Z Life Sciences [q-bio]Journal articles %X Background/Aim: Multiple myeloma (MM) is characterized by high production of immunoglobulins resulting in a constant source of endoplasmic reticulum (ER)-stress. Mesencephalic astrocyte-derived neurotrophic factor (MANF) was identified as a possible circulating biomarker that could help in monitoring ER-stress mediated diseases. Materials and Methods: To assess the relevance of MANF in MM, we performed in silico and in vitro analysis in malignant cell lines including the myeloma cell line RPMI 8226. Serum MANF concentration was compared between healthy subjects (n=60), patients with MM (n=68), or those with monoclonal gammopathy of undetermined significance (MGUS) (n=73). Results: MANF mRNA expression was upregulated in the RPMI 8226 cell line, and higher secretion of MANF was measured in RPMI 8226 supernatant. Serum MANF levels were not significantly different between MM or MGUS patients and those in age-and sex-matched healthy controls. Conclusion: MANF was not validated as a biomarker of interest in MM patients. Its potential implication in myeloma pathogenesis should be investigated. %G English %L hal-03574521 %U https://u-picardie.hal.science/hal-03574521 %~ UNIV-RENNES1 %~ UNIV-PICARDIE %~ FNCLCC %~ MARQUIS %~ OSS %~ UR1-UFR-SVE %~ UR1-HAL %~ UR1-SDV %~ TEST-UR-CSS %~ UNIV-RENNES %~ UR1-BIO-SA %~ U-PICARDIE %~ CHIMERE %~ MP3CV %~ HEMATIM