%0 Journal Article %T The Cell Wall Arabinose-Deficient Arabidopsis thaliana Mutant murus5 Encodes a Defective Allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 %+ Biologie des Plantes et Innovation - UR UPJV 3900 (BIOPI) %+ Department of Botany and Plant Pathology %A Dugard, Christopher K. %A Mertz, Rachel A. %A Rayon, Catherine %A Mercadante, Davide %A Hart, Christopher %A Benatti, Matheus R. %A Olek, Anna T. %A Sanmiguel, Phillip J. %A Cooper, Bruce R. %A Reiter, Wolf-Dieter %A Mccann, Maureen C. %A Carpita, Nicholas C. %< avec comité de lecture %@ 0032-0889 %J Plant Physiology %I Oxford University Press ; American Society of Plant Biologists %V 171 %N 3 %P 1905-1920 %8 2016 %D 2016 %R 10.1104/pp.15.01922 %Z Life Sciences [q-bio]Journal articles %X Traditional marker-based mapping and next-generation sequencing was used to determine that the Arabidopsis (Arabidopsis thaliana) low cell wall arabinose mutant murus5 (mur5) encodes a defective allele of REVERSIBLY GLYCOSYLATED POLYPEPTIDE2 (RGP2). Marker analysis of 13 F2 confirmed mutant progeny from a recombinant mapping population gave a rough map position on the upper arm of chromosome 5, and deep sequencing of DNA from these 13 lines gave five candidate genes with G -> A (C -> T) transitions predicted to result in amino acid changes. Of these five, only insertional mutant alleles of RGP2, a gene that encodes a UDP-arabinose mutase that interconverts UDP-arabinopyranose and UDP-arabinofuranose, exhibited the low cell wall arabinose phenotype. The identities of mur5 and two SALK insertional alleles were confirmed by allelism tests and overexpression of wild-type RGP2 complementary DNA placed under the control of the 35S promoter in the three alleles. The mur5 mutation results in the conversion of cysteine-257 to tyrosine-257 within a conserved hydrophobic cluster predicted to be distal to the active site and essential for protein stability and possible heterodimerization with other isoforms of RGP. %G English %L hal-03610851 %U https://u-picardie.hal.science/hal-03610851 %~ UNIV-ARTOIS %~ UNIV-PICARDIE %~ UNIV-LITTORAL %~ UNIV-LILLE %~ INRAE %~ U-PICARDIE %~ BIOPI-UPJV %~ BIOECOAGRO-UPJV %~ UNIV-CATHOLILLE %~ JUNIA