%0 Journal Article %T High resolution MALDI-TOF-MS and MS/MS: Application for the structural characterization of sulfated oligosaccharides %+ Laboratoire de Glycochimie, des Antimicrobiens et des Agro-ressources - UMR CNRS 7378 (LG2A ) %A Lesur, David %A Duhirwe, Gilbert %A Kovensky, Jose %< avec comité de lecture %@ 1469-0667 %J European Journal of Mass Spectrometry %I IM Publications %V 25 %N 5 %P 428-436 %8 2019 %D 2019 %R 10.1177/1469066719851438 %Z Chemical SciencesJournal articles %X Sulfated oligosaccharides are involved in important biological events that are often modulated by specific sequences and sulfation patterns, but their structural analysis remains challenging. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) analysis of three different sulfated oligosaccharides (Fondaparinux, the octasulfated pentasaccharide, a disulfated heparin-derived tetrasaccharide 1, and an octasulfated maltoheptaose) 2 was performed using the 2-(4-hydroxyphenylazo)benzoic acid-tetramethylguanidinium (HABA-TMG(2)) matrix. High resolution mass spectrometry of the main ions observed was successful, and this was complemented by tandem mass spectrometry (MS/MS) analysis for structural assessment. Despite sulfate losses, fully sulfated molecular ions were observed and these allowed the determination of oligosaccharide structures: UA-GlcNAc-UA(2S)-AnhMan(6S) for compound 1 and (Glc6S)(6)-Glc (1S,6S) for compound 2. %G English %L hal-03613822 %U https://u-picardie.hal.science/hal-03613822 %~ CNRS %~ UNIV-PICARDIE %~ INC-CNRS %~ U-PICARDIE %~ LG2A %~ TEST2-HALCNRS