%0 Journal Article %T Caffeine citrate effects on gastrointestinal permeability, bacterial translocation and biochemical parameters in newborn rats after long-term oral administration %+ Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX) %A Bouabsa, Foufa %A Touil, Aicha Tir %A Al Zoubi, Mazhar Salim %A Chellia, Nadia %A Leke, Andre %A Meddah, Boumediene %< avec comité de lecture %@ 1973-798X %J Mediterranean Journal of Nutrition and Metabolism %I IOS Press %V 15 %N 3 %P 307-321 %8 2022 %D 2022 %R 10.3233/MNM-211544 %K Caffeine %K neonatal life %K intestinal permeability %K bacterial translocation %K oxidative stress parameters %Z Life Sciences [q-bio]/Human health and pathologyJournal articles %X BACKGROUND: Caffeine is a potent central and respiratory acting agent used in neonatology to treat apnea in premature newborns. OBJECTIVE: This study investigates the effects of caffeine orally administered to newborn rats on gastrointestinal permeability, bacterial translocation and different biochemical parameters. METHODS: Newborn rats were divided into different groups (N= 06). The treated newborn rats were orally administered with standard caffeine doses (12 mg/kg per day), and the control groups received a placebo. The animals were weighed daily until sacrifice. Blood samples, mesenteric lymph nodes (MLN) and organs were aseptically collected. Furthermore, different biochemical (D-Lactate) and oxidative stress biomarkers (MDA, CAT, SOD and GSH) were examined. Microbiological analyses were performed to assess microbiota alterations and bacterial translocation. RESULTS: Preliminary results showed that caffeine administration decreased the level of bacterial translocation over time. The treatment reduced plasma D-lactate levels (p < 0.05). Additionally, caffeine induced a disturbance in the concentrations of biochemical parameters and oxidative stress biomarkers. Indeed, liver enzymes (AST and ALT) were significantly (p < 0.05) risen after caffeine treatment. Glutathione (GSH) levels were significantly higher in caffeine treated groups (75.12 +/- 0.32; 51.98 +/- 1.12 U/mg; p < 0.05) comparing to control ones (40.82 +/- 0.25; 42.91 +/- 0.27 U/mg; p < 0.05) in the ileum and the colon, respectively. CONCLUSIONS: Thus, besides improving gastrointestinal permeability, our data show that caffeine has beneficial effects on the intestinal antioxidant system. %G English %L hal-03791159 %U https://u-picardie.hal.science/hal-03791159 %~ INERIS %~ UNIV-PICARDIE %~ U-PICARDIE %~ PERITOX