%0 Journal Article %T Perigestational exposure of a combination of a high-fat diet and pesticide impacts the metabolic and microbiotic status of dams and pups; a preventive strategy based on prebiotics %+ Périnatalité et Risques Toxiques - UMR INERIS_I 1 (PERITOX) %+ Transformations et Agro-ressources (UT&A) %+ Université frères Mentouri Constantine I (UMC) %+ CHU Amiens-Picardie %A Djekkoun, Narimane %A Depeint, Flore %A Guibourdenche, Marion %A Sabbouri, Hiba El Khayat Et %A Corona, Aurélie %A Rhazi, Larbi %A Gay-Queheillard, Jerome %A Rouabah, Leila %A Biendo, Maurice %A Al-Salameh, Abdallah %A Lalau, Jean-Daniel %A Bach, Véronique %A Khorsi-Cauet, Hafida %< avec comité de lecture %@ 1436-6207 %J European Journal of Nutrition %I Springer Verlag %V 62 %N 3 %P 1253-1265 %8 2023 %D 2023 %R 10.1007/s00394-022-03063-y %K Prebiotic %K Dysmetabolism %K High-fat diet %K Microbiota %K Perigestational period %K Pesticide %Z Life Sciences [q-bio]/Human health and pathologyJournal articles %X Purpose: Metabolic changes during the perinatal period are known to promote obesity and type-2 diabetes in adulthood via perturbation of the microbiota. The risk factors for metabolic disorders include a high-fat diet (HFD) and exposure to pesticide residues. The objective of the present study was to evaluate the effects of perigestational exposure to a HFD and chlorpyrifos (CPF) on glycemia, lipid profiles, and microbial populations in Wistar dams and their female offspring. We also tested a preventive strategy based on treatment with the prebiotic inulin.Methods: From 4 months before gestation to the end of the lactation period, six groups of dams were exposed to either a standard diet, a HFD alone, CPF alone, a combination of a HFD and CPF, and/or inulin supplementation. All female offspring were fed a standard diet from weaning to adulthood. We measured the impacts of these exposures on glycemia, the lipid profile, and the microbiota (composition, metabolite production, and translocation into tissues).Results: HFD exposure and CPF + HFD co-exposure induced dysmetabolism and an imbalance in the gut flora in both the dams and the female offspring. Inulin mitigated the impact of exposure to a HFD alone but not that of CPF + HFD co-exposure.Conclusion: Our results provide a better understanding of the complex interactions between environmental pollutants and diet in early life, including in the context of metabolic diseases. %G English %L hal-03901249 %U https://u-picardie.hal.science/hal-03901249 %~ UNIV-PICARDIE %~ COMUE-NORMANDIE %~ UNILASALLE %~ U-PICARDIE %~ PERITOX