BK virus genotypes and humoral response in kidney transplant recipients with BKV associated nephropathy - Université de Picardie Jules Verne Accéder directement au contenu
Article Dans Une Revue Transplant Infectious Disease Année : 2023

BK virus genotypes and humoral response in kidney transplant recipients with BKV associated nephropathy

Etienne Brochot

Résumé

Background: Among kidney transplant recipients (KTR) with BK virus associated nephropathy (BKVN), BKV genotypes' evolution and anti-BKV humoral response are not well established. We aim to analyze BKV replication and genetic evolution following transplantation, and characterize concomitant anti-BKV-VP1 humoral response. Methods: We retrospectively analyzed 32 cases of biopsy-proven BKVN. Stored plasma and kidney biopsies were tested for BKV viral load, and VP1 sequencing performed on positive samples. BKV-VP1 genotype-specific neutralizing antibodies (NAbs) titers were determined at transplantation and BKVN. Results: At the time of BKVN diagnosis, BKV viral load was 8.2 log10 IU/106 cells and 5.4 log10 IU/mL in kidney and plasma, respectively. VP1 sequencing identified the same BKV-subtype in both compartments in 31/32 cases. At the time of transplantation, 8/20 (40%) of biopsies tested positive for BKV detection, whereas concomitant BKV viremia was negative. VP1 sequencing identified a different subtype compared to BKVN in 5/6 of these samples. This was confirmed following transplantation: 8 patients had a BKV+ biopsy before BKV viremia, and VP1 sequencing identified a different subtype compared to BKVN in all of them. After the onset of BKV viremia and prior to BKVN diagnosis, the BKV subtype in BKV+ plasma and kidney biopsy was the same as the one isolated at BKVN. BKV-VP1 NAbs titers were significantly higher at the time of BKVN compared to transplantation (p = .0031), with similar titers across genotypes. Conclusion: Altogether, our data suggest that among some KTR with BKVN, the BKV genotype from the donor may not be responsible for BKVN pathogenesis.
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hal-03980734 , version 1 (06-06-2023)

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Julien Gras, Marie Laure Nere, Marie Noëlle Peraldi, Lucie Bonnet-Madin, Maud Salmona, et al.. BK virus genotypes and humoral response in kidney transplant recipients with BKV associated nephropathy. Transplant Infectious Disease, 2023, ⟨10.1111/tid.14012⟩. ⟨hal-03980734⟩
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