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Article Dans Une Revue International Journal of Biological Macromolecules Année : 2016

Molecular investigations of flaxseed mucilage polysaccharides

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The molecular properties of flaxseed mucilage were determined using a multi-angle laser light scattering (MALLS) detector coupled on-line to size exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4). Water and salt solution were tested as mobile phases. The SEC-MALLS method gave partial information and enabled molecular characterization of disaggregated mucilage molecules. Regardless of the eluent used, the observed M-w ranged from about 1.6 x 10(6) to more than 10 x 10(6) g/mol for mucilage polysaccharides. The AF4-MALLS system enabled a complete analysis of mucilage carbohydrate aggregates in water, in which two populations were satisfactorily separated. The molecular weight distribution (MWD) of molecules ranged from 1.5 x 10(6) to more than 4 x 10(8) g/mol. Experiments showed that the conformational structure of mucilage molecules was strongly influenced by ionic strength. Mucilage carbohydrates exhibited a spherical and compact structure in NaCl solution while they displayed a random-coil conformation in water. (C) 2016 Elsevier B.V. All rights reserved.
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Romain Roulard, Emmanuel Petit, Francois Mesnard, Larbi Rhazi. Molecular investigations of flaxseed mucilage polysaccharides. International Journal of Biological Macromolecules, 2016, 86, pp.840-847. ⟨10.1016/j.ijbiomac.2016.01.093⟩. ⟨hal-03609614⟩
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