Molecular investigations of flaxseed mucilage polysaccharides - Université de Picardie Jules Verne Accéder directement au contenu
Article Dans Une Revue International Journal of Biological Macromolecules Année : 2016

Molecular investigations of flaxseed mucilage polysaccharides


The molecular properties of flaxseed mucilage were determined using a multi-angle laser light scattering (MALLS) detector coupled on-line to size exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4). Water and salt solution were tested as mobile phases. The SEC-MALLS method gave partial information and enabled molecular characterization of disaggregated mucilage molecules. Regardless of the eluent used, the observed M-w ranged from about 1.6 x 10(6) to more than 10 x 10(6) g/mol for mucilage polysaccharides. The AF4-MALLS system enabled a complete analysis of mucilage carbohydrate aggregates in water, in which two populations were satisfactorily separated. The molecular weight distribution (MWD) of molecules ranged from 1.5 x 10(6) to more than 4 x 10(8) g/mol. Experiments showed that the conformational structure of mucilage molecules was strongly influenced by ionic strength. Mucilage carbohydrates exhibited a spherical and compact structure in NaCl solution while they displayed a random-coil conformation in water. (C) 2016 Elsevier B.V. All rights reserved.
Fichier non déposé

Dates et versions



Romain Roulard, Emmanuel Petit, Francois Mesnard, Larbi Rhazi. Molecular investigations of flaxseed mucilage polysaccharides. International Journal of Biological Macromolecules, 2016, 86, pp.840-847. ⟨10.1016/j.ijbiomac.2016.01.093⟩. ⟨hal-03609614⟩
31 Consultations
0 Téléchargements



Gmail Mastodon Facebook X LinkedIn More