Impacts of perigestational exposure to Chlorpyrifos (CPF) and High-Fat Diet (HFD) on ileum contractility in male rats. - Université de Picardie Jules Verne Accéder directement au contenu
Poster De Conférence Année : 2019

Impacts of perigestational exposure to Chlorpyrifos (CPF) and High-Fat Diet (HFD) on ileum contractility in male rats.

Résumé

Background: Chlorpyrifos (CPF) is an acetylcholinesterase (AchE)-inhibiting organophosphorus insecticide. Exposure to CPF is harmful during the perinatal period. Such developmental perturbations are linked to further disorders at adulthood through fetal programming. These disturbances are related to unbalanced diet and environmental pollutants. This study established the effects of perigestational exposure to CPF and to high-fat diet (HFD) on the contractility of smooth muscle in adult rats. Methods: 4 groups of 4 female rats were exposed during 4 months before and later during gestation and lactation periods to CPF (1 mg/kg bw/day vs vehicle) with or without HFD. After being sacrificed at the age of 60 postnatal days (PND60), ileal smooth muscle strips were used for in vitro contractility measurements in an isolated organ bath system, AchE activity assessment, and genes expression evaluation (n=7-10 per group). Results: At PND60, CPF exposure induced an increase in the ileal longitudinal and circular muscles contractility. The circular muscle thickness was also increased. Indeed, CPF exposure was associated with greater expression of the tachykinin (substance P) mRNA and the muscarinic M2 acetylcholine receptor mRNA in the ileum. Furthermore, the expression of tachykinin NK1 receptor mRNA was increased in HFD group compared to controls. The exposure to either CPF and/or HFD induced a decrease in AchE activity in the ileum. Conclusion: The perigestational exposure to CPF is associated with increased ileal muscle contractility at adulthood due to lower AchE activity via cholinergic and non-cholinergic mechanisms. Ileal contractility was not affected in the rats of HFD-fed mothers despite elevated NK1 receptor expression and reduced AchE activity levels. Integrating this study helps in preventing chronic gut diseases in adults.
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Dates et versions

hal-04396401 , version 1 (15-01-2024)

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  • HAL Id : hal-04396401 , version 1

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Hiba El Khayat El Sabbouri, Marion Guibourdenche, Walaa Darwiche, Narimane Djekkoun, Véronique Bach, et al.. Impacts of perigestational exposure to Chlorpyrifos (CPF) and High-Fat Diet (HFD) on ileum contractility in male rats.. 4th Biennial Meeting of the European Society of Neurogastroenterology and Motility (NeuroGASTRO), Sep 2019, LISBON, Portugal. ⟨hal-04396401⟩
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