Impacts of Perigestational Exposure to Chlorpyrifos And High-Fat Diet on Ileum Contractility in Male Rats at Early Adulthood - Université de Picardie Jules Verne Accéder directement au contenu
Article Dans Une Revue jpdm Année : 2021

Impacts of Perigestational Exposure to Chlorpyrifos And High-Fat Diet on Ileum Contractility in Male Rats at Early Adulthood

Résumé

Background: The perinatal period is characterized by strong plasticity and higher individual sensitivity to environmental factors through fetal programming. An association of junk food and early exposure to pesticide residues can be involved in metabolic disruption and increased pathologies including obesity. Aims: This study established the effects of perigestational exposure to a widely used organophosphate pesticide, Chlorpyrifos (CPF), and high-fat diet (HFD) on the contractility of ileal smooth muscle in adult rats. Methods: Four groups of four female rats were exposed during 4 months before and later during gestation and lactation periods to CPF (1 mg/kg bw/day vs. vehicle) with or without HFD. After being sacrificed at the age of 60 postnatal days (PND60), ileal smooth muscle strips were used for in vitro contractility measurements. Other ileal segments were used for AChE activity assessment, gene expression measurement, and histological analysis. KeyPoints: • Perigestational exposure to CPF increased ileal muscle contractility at adulthood. • The altered ileal contraction involves cholinergic and non-cholinergic mechanisms. • Offspring from HFD-fed mothers showed elevated NK1 receptor expression. Keywords: Chlorpyrifos; High-Fat Diet; Maternal Exposure; Ileum; Contractility Results: At PND60, CPF exposure increased the ileal longitudinal and circular muscles contractility. The circular muscle thickness was also increased. CPF exposure was associated with greater expression of the tachykinin (substance P) and the muscarinic M2 acetylcholine receptor mRNA but lower expression of calmodulin mRNA in the ileum. The expression of tachykinin NK1 receptor mRNA was increased in HFD group compared to controls. The exposure to either CPF and/or HFD induced a decrease in the ileal AChE activity. Conclusions: Despite the lack of direct exposure post-weaning, early life exposure to CPF and /or HFD programs the ileal homeostasis and increases the risk of altered gut contractile function at early adulthood.
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hal-04410994 , version 1 (22-01-2024)

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Hiba El Khayat El Sabbouri, Marion Guibourdenche, Walaa Darwiche, Wissam Joumaa, Narimane Djekkoun, et al.. Impacts of Perigestational Exposure to Chlorpyrifos And High-Fat Diet on Ileum Contractility in Male Rats at Early Adulthood. jpdm, 2021, ⟨10.17303/jpdm.2021.4.101⟩. ⟨hal-04410994⟩

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