Antiallergic Effect of the Alpha‐Cyclodextrin Moringin Complex in Rat Basophilic Leukaemia (RBL‐2H3) Cell Line
Résumé
Allergic diseases (ADs) are a major concern when it comes to public well‐being. Moringa oleifera Lam is a tropical plant that is used in traditional medicine due to the presence of isothiocyanate. The present study investigated the antiallergic properties of 4‐( α ‐L‐rhamnopyranosyloxy)‐benzyl isothiocyanate or moringin isolated from Moringa oleifera seeds in the form of alpha‐cyclodextrin‐moringin ( α ‐CD/MG) complex on rat basophilic leukaemia (RBL‐2H3) cell line at both the early and late stages of an allergic reaction. The α ‐CD/MG complex was initially elucidated using nuclear magnetic resonance (NMR) followed by the 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium inner salt proliferation assay to evaluate the cytotoxicity and cell viability with respect to ketotifen fumarate (KF) and α ‐CD/MG. The release of beta‐hexosaminidase ( β ‐hexosaminidase) and histamine was used to determine the level of inhibition in the early stage while the suppression of the release of prostaglandin (PGD2), tumour necrosis factor‐alpha (TNF‐ α ), and interleukin (IL‐4) was considered in the late stage. Higher concentrations of α ‐CD/MG (5 μ M, p < 0.001) in mast cell degranulation significantly inhibited the expression of β ‐hexosaminidase, histamine, TNF‐ α , PGD2, and IL‐4 in both the early and late stages. Thus, α ‐CD/MG can potentially be developed as an antiallergic drug as it has the ability to inhibit allergic responses in the late and early stages.