Article Dans Une Revue Medical Mycology Année : 2025

Development of in-house ELISA for Scedosporium/Lomentospora serodiagnosis

1 LCE - Laboratoire Chrono-environnement (UMR 6249)
2 CNR-E - Centre National de Référence Echinococcoses
3 CHRU Besançon - Centre Hospitalier Régional Universitaire de Besançon
4 Hôpital Necker - Enfants Malades [AP-HP]
5 CHU - BREST - Hôpital de la Cavale Blanche - CHRU Brest
6 CHRU Brest - Centre Hospitalier Régional Universitaire de Brest
7 CHU Amiens-Picardie
8 Laboratoire de parasitologie et de mycologie médicales [CHU Amiens]
9 UNIROUEN - Université de Rouen Normandie
10 Département de Microbiologie [CHU Rouen]
11 UNIROUEN UFR Santé - UNIROUEN - UFR Santé
12 IICiMed - Cibles et Médicaments des Infections et de l'Immunité - UR 1155
13 CHU Nantes - Centre Hospitalier Universitaire de Nantes = Nantes University Hospital
14 Service de Parasitologie et Mycologie [CHU Toulouse]
15 CNR Paludisme - Centre National de Référence Paludisme [AP-HP Hôpital Bichat-Claude Bernard]
16 Hôpital Avicenne [AP-HP]
17 UCBL - Université Claude Bernard Lyon 1
18 HCL - Hospices Civils de Lyon
19 Service de Parasitologie-Mycologie [CHRU LIlle]
20 UGSF - Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576
21 CERPOP - Centre d'Epidémiologie et de Recherche en santé des POPulations
22 CHU Pitié-Salpêtrière [AP-HP]
23 URePSSS - Unité de Recherche Pluridisciplinaire Sport, Santé, Société (URePSSS) - ULR 7369 - ULR 4488
24 ICAT - SFR UA 4208 Interactions Cellulaires et Applications Thérapeutiques
25 IRF - Infections Respiratoires Fongiques
26 CHU Angers - Centre Hospitalier Universitaire d'Angers
27 Unité de Parasitologie-Mycologie [CHU HEGP]
28 UPCité - Université Paris Cité
29 DYNAMIC - Dynamic Microbiology - EA 7380
Taieb Chouaki

Résumé

Scedosporium/Lomentospora species are ubiquitous molds that can cause deep-seated infections and allergic bronchopulmonary mycoses (ABPM). Serodiagnosis is currently performed by immunoprecipitation techniques (IP), which are time-consuming and lack reproducibility. In addition, as antigenic extracts for these fungi are not commercially available, many centers stopped performing this analysis. Therefore, there is a need for automated quantitative alternatives, such as ELISA. The aim of this study was to develop an ELISA for serodiagnosis of Scedosporium/Lomentospora infections. All sera received for Scedosporium/Lomentospora serodiagnosis expertise from April 2022 to February 2024 were tested in parallel using IP and an in-house ELISA with antigenic extracts from both S. apiospermum and L. prolificans. Clinical and biological data such as positive culture, total IgE level and final diagnosis retained were also collected prospectively. The concordance between techniques was calculated, with chi² tests performed to investigate the correlation between ELISA and culture results or final diagnosis. We tested 58 serum samples from 41 different patients. The concordance between IP and ELISA was 64% for S. apiospermum and 62% for L. prolificans. ELISA results obtained with S. apiospermum antigen extract were significantly correlated with culture results (p < 0.001, chi² test). ELISA was also more effective than IP to diagnose ABPM. The Scedosporium/Lomentospora ELISA gave satisfactory results, particularly for S. apiospermum. Further validation on a larger cohort is required implementing this ELISA for routine practice instead of IP. In addition, studies should be conducted on purified native proteins or combination with recombinant antigens to improve standardization.

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Dates et versions

hal-05185259 , version 1 (24-07-2025)

Identifiants

Citer

Coralie Barrera, Marie-Elisabeth Bougnoux, Claire Hoffmann, Céline Damiani, Damien Costa, et al.. Development of in-house ELISA for Scedosporium/Lomentospora serodiagnosis. Medical Mycology, 2025, 63 (8), pp.myaf067. ⟨10.1093/mmy/myaf067⟩. ⟨hal-05185259⟩
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